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Spermatogenesis & Semen

Animal Breeding

Pensacola Jr College

Puberty in Males

w    LH pulses stimulate maturation of Leydig cells

w    Testosterone production begins

w    Sperm formed in seminiferous tubules

w    2ndary sex characteristics

Spermatogenesis

w    Occurs constantly from puberty on, groups of cells start 3 step cycle all the time (except seasonal breeders)

w    Cells in one region of tubule start process at same time, creating spermatogenic wave

Steps of Spermatogenesis

w    Spermatocytogenesis = formation of the spermatocytes

w    Meiosis = formation of the spermatids

w    Spermiogenesis = metamorphosis into sperm cell

 

1. Spermatocytogenesis

w    Begin with spermatogonia = round stem cells on basement membrane of seminiferous tubules

w    Undergo mitosis

w    Each produces two diploid primary spermatocytes

Spermatocytogenesis

2. Meiosis

Meiosis

3. Spermiogenesis

w    Round spermatids attach to sertoli cells and go through changes in form

w    Nucleus moved to one end

w    Mitochondria packed in one area

w    Cytoplasm lost

w    Flagellum forms

Spermiogenesis

Spermiogenesis

Sperm Structure

w    Head: has cell membrane, acrosome (enzyme bag), nucleus

w    Midpiece: has cytoplasmic droplet, cell membrane, tightly packed mitochondria

w    Tail: flagellum with contractile fibers

 

Sperm (SEM)

Sperm Maturation

w    In the epididymis:

    Gain motility

    Gain fertility

    Lose cytoplasmic droplet

w    In female reproductive tract:

    Final maturation process

Semen

w    Fluids produced by male accessory sex glands to maintain sperm cell homeostasis

w    Ions (esp. Na and Cl)

w    Buffers (esp. bicarbonate)

w    Energy sources (esp. fructose and sorbitol)

w    Other unique compounds